Premium
Measuring k L a with randomly pulsed dynamic method
Author(s) -
Gauthier Line,
Thibault Jules,
LeDuy Anh
Publication year - 1991
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260370914
Subject(s) - reproducibility , reliability (semiconductor) , transfer function , analytical chemistry (journal) , volumetric flow rate , materials science , chromatography , chemistry , biological system , mechanics , thermodynamics , engineering , physics , biology , power (physics) , electrical engineering
Abstract This reports on the determination of the overall oxygen transfer coefficient in a mechanically agitated vessel using a randomly pulsed dynamic method. This method consists in exciting the system by randomly switching the inlet gas stream with air or nitrogen with an identical volumetric flow rate. A pseudo‐random binary sequence was used. This procedure is routinely used in process control for the identification of system's transfer function. The pulsed dynamic method gives good reliability (as compared with the traditional gassing‐out method) and reproducibility in water. However, further improvement is needed before it can be used to monitor on‐line the k L a during a fermentation.