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Enzymatic cellulose hydrolysis in an attrition bioreactor combined with an aqueous two‐phase system
Author(s) -
Tjerneld Folke,
Persson Ingrid,
Lee James M.
Publication year - 1991
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260370912
Subject(s) - bioreactor , chromatography , cellulose , cellulase , hydrolysis , aqueous solution , enzymatic hydrolysis , polyethylene glycol , chemistry , aqueous two phase system , dilution , substrate (aquarium) , dextran , biochemistry , organic chemistry , biology , physics , thermodynamics , ecology
Cellulose was hydrolyzed in the attrition bioreactor (ABR) with enzyme recycling by employing an aqueous two‐phase system (composed of dextran and polyethylene glycol) and an ultrafiltration unit. The ABR combines wet ball milling and enzymatic hydrolysis in one process step. The cellulase enzymes were more stable in the two‐phase system than in the normal buffer solution. With the initial substrate concentration (Solka Floe BW200) of 40 g/L and intermittent addition of cellulose, sugar was semicontinuously produced at dilution rates of 0.06 h −1 and productivities of 2.1 g/L h, which is approximately a 10‐fold increase of the previously reported values performed in a regular stirred reactor with an aqueous two‐phase system. The conversion of the substrate was 86%.