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Affinity chromatography as a rapid and convenient method for purification of fungal laccases
Author(s) -
Rogalski J.,
WojtasWasilewska M.,
Apalovič R.,
Leonowicz A.
Publication year - 1991
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260370812
Subject(s) - laccase , chromatography , trametes versicolor , immobilized enzyme , chemistry , affinity chromatography , porous glass , enzyme , porosity , organic chemistry
A rapid and convenient method for graduation, isolation, and purification of laccase from Trametes versicolor and Fomes fomentarius culture fluids was developed. For purification affinity chromatography on syringyl‐ and vanillyl‐controlled porosity glass (CPG) columns was applied. The purified laccase of F. fomentarius was immobilized on porous glass. Some properties of the immobilized enzyme in comparison to the free one are discussed.