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High‐concentration cultivation of Lactococcus cremoris in a cell‐recycle reactor
Author(s) -
Bibal Bernard,
Vayssier Yves,
Goma Gérard,
Pareilleux Alain
Publication year - 1991
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260370809
Subject(s) - dilution , fermentation , lactic acid , lactose , biomass (ecology) , chemistry , food science , filtration (mathematics) , lactic acid fermentation , chromatography , lactococcus , nutrient , biochemistry , lactococcus lactis , biology , bacteria , physics , genetics , statistics , mathematics , organic chemistry , agronomy , thermodynamics
High‐cell concentration cultivation of Lactococcus cremoris , a homofermentative lactic acid producer, in a cell‐recycle fermentor is described. Cross‐flow filtration allowing continuous removal of the inhibitory metabollte, the influence of dilution rate on growth was investigated in total or partial cell‐recycle cultures. The dependence of growth characteristics on operating conditions was identified and quantified using lactose as the carbon source. Growth kinetics could be described by both lactate removal efficiency and nutrient availability. Based on physiological observations, biomass and lactic acid productivities were predicted in partial cell‐recycle cultures.