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Translation of immobilized genetic information by yeast cell‐free protein synthesizing system
Author(s) -
Kobatake Eiry,
Ikariyama Yoshihito,
Aizawa Masuo
Publication year - 1991
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260370806
Subject(s) - yeast , translation (biology) , computational biology , chemistry , biochemistry , translation system , protein biosynthesis , cell free system , biochemical engineering , biology , microbiology and biotechnology , enzyme , gene , messenger rna , engineering , in vitro
A cell‐free protein‐synthesizing system for the purpose of specific genetic information translation was constructed: ribosome, t‐RNA, and enzymes extracted from yeast cells were combined with an immobilized mRNA. The soluble fraction mixed with energy sources and amino acids was incubated with the immobilized mRNA such as poly(U), yeast mRNA, and myeloma mRNA to incorporate [ 3 H]phenylalanine into polypeptides of de novo synthesis. By supplying amino acids to these protein‐synthesizing systems, amino acid incorporation was ascertained. Lower efficiency of the incorporation in the immobilized system than that of the homogeneous system was mainly attributed to the heterogeneous reaction where the mass‐transfer process is diffusion limited. Results obtained show the possibility of a system for specific translation of a desired genetic code.