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A novel magnetic silica support for use in chromatographic and enzymatic bioprocessing
Author(s) -
Goetz Victor,
Remaud Magali,
Graves David J.
Publication year - 1991
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260370704
Subject(s) - chromatography , polymer , adsorption , chemical engineering , fluidized bed , immobilized enzyme , porosity , chemistry , monosaccharide , magnetic nanoparticles , magnetite , composite number , yield (engineering) , cationic polymerization , invertase , magnetic separation , materials science , nanoparticle , sucrose , enzyme , composite material , organic chemistry , nanotechnology , metallurgy , engineering
A limited number of support matrices have so far been developed for use in magnetically stabilized fluidized bed (MSFB) applications. We have developed a versatile magnetic silica support which can be derivatized readily for both adsorption chromatography and enzyme immobilization by well‐known techniques. A magnetic pellicular bead is prepared by electrostatically depositing alternating layers of colloidal silica and cationic polymer onto macroscopic nickel core particles. The polymer is then burned out and the silica partially sintered to yield a porous shell with 5–80 m 2 /g of surface area. This magnetic composite was tested as a support for immobilizing invertase. Sucrose was continuously converted to its component monosaccharides with nearly constant activity over the first 8 days and retention of 50% of initial activity after 25 days.