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The effect of pore‐size distribution on the binding of proteins to porous resin beads
Author(s) -
Hasegawa Masanori,
Kitano Hiromi
Publication year - 1991
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260370703
Subject(s) - porosity , chemistry , chemical engineering , distribution (mathematics) , porous medium , materials science , biophysics , organic chemistry , biology , mathematics , engineering , mathematical analysis
The effect of the distribution of pore radii in the resin beads on protein binding was taken into account to analyze the elution profiles of proteins from the polymer‐packed column obtained by repetitive injection method. By assuming that the distribution of pore radii in the resin beads is logarithmic Gaussian, the theoretical curves obtained agreed well with the experimental data.