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Hydrolysis of inulin: A kinetic study of the reaction catalyzed by an inulinase from Aspergillus ficuum
Author(s) -
Carniti Paolo,
Beltrame Pier Luigi,
Guardione Donatella,
Focher Bonaventura,
Marzetti Annamaria
Publication year - 1991
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260370612
Subject(s) - inulinase , inulin , chemistry , invertase , hydrolysis , catalysis , activation energy , enzyme , chromatography , biochemistry , organic chemistry
A kinetic study of the hydrolysis of inulin was performed by using as catalyst a commercial inulinase from Aspergillus ficuum. The reaction was studied carrying out initial rate as well as time course measurements. Both inulinase and invertase activities of the enzyme were taken into account, and the corresponding kinetic parameters were determined in the temperature range 30‐50°C. The activation energies of the turnover constant for inulinase and invertase activities were found to be similar (56–57 kJ · mol −1 ). The ratio S/I of invertase to inulinase activity was 1.6 regardless of temperature. The thermal degradation of the enzyme was also investigated up to 70°C, and an activation energy of 350‐370 kJ · mol −1 was evaluated.