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In situ recalibration of probes of dissolved gas concentrations in bioreactors without effect on metabolism and growth
Author(s) -
Iversen J. J. Lønsmann
Publication year - 1991
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260370508
Subject(s) - anabaena variabilis , klebsiella oxytoca , oxygen , bioreactor , substrate (aquarium) , clark electrode , in situ , oxygen tension , chemistry , galvanic cell , environmental chemistry , analytical chemistry (journal) , chromatography , electrode , cyanobacteria , biology , biochemistry , ecology , bacteria , genetics , organic chemistry , escherichia coli , klebsiella pneumoniae , gene , electrolyte
This article describes a general method for the in situ recalibration of a probe for dissolved gas concentrations without changing any parameter but the gas composition. Recalibration of an oxygen probe is used as an example. The recalibration procedure can be used for any probe, provided the culture's rate of metabolism and growth is independent of the substrate concentration in the range being measured by the probe during recalibration. The method was tested using a galvanic oxygen electrode in an oxygen‐consuming culture of Klebsiella oxytoca and an oxygen‐evolving culture of Anabaena variabilis. The recalibration caused no detectable effect on the metabolism and growth of the cultures, provided the dissolved oxygen tension was above 3μ M .