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Lewis cell studies to determine reactor design data for two‐liquid‐phase bacterial and enzymic reactions
Author(s) -
Woodley J. M.,
Brazier A. J.,
Lilly M. D.
Publication year - 1991
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260370207
Subject(s) - pseudomonas putida , chemistry , biocatalysis , aqueous two phase system , hydrolysis , aqueous solution , toluene , substrate (aquarium) , esterase , phase (matter) , batch reactor , kinetics , organic chemistry , chromatography , chemical engineering , catalysis , reaction mechanism , enzyme , oceanography , physics , quantum mechanics , engineering , geology
Substrate transfer rates from organic to aqueous phases were measured in the presence and absence of biocatalyst in the reaction medium, using modified Lewis cells. These measurements, in combination with intrinsic aqueous phase biocatalytic reaction kinetics, were used to confirm that benzyl acetate hydrolysis by pig liver esterase and toluene oxidation by a strain of Pseudomonas putida occur uniformly throughout the bulk of the aqueous phase. Such data may be used to provide a basis for two‐liquid‐phase biocatalytic reactor design.