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Effects of solvent molecular toxicity and microenvironment composition on the Δ 1 dehydrogenataon activity of Arthrobacter simplex cells
Author(s) -
Pinheiro H. M.,
Cabral J. M. S.
Publication year - 1991
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260370202
Subject(s) - chemistry , solvent , dehydrogenation , solubility , chloroform , aqueous solution , organic chemistry , chromatography , catalysis , nuclear chemistry
Solvent selection tests were carried out for the Δ 1 dehydrogenation of 6‐α‐methylhydrocortisone‐21‐acetate by Arthrobacter simplex cells in the presence of organic solvents. Solubility limits were determined for substrate and product in dry and water‐saturated solvents and solvent mixtures. Molecular toxicity levels were estimated by measuring the dehydrogenation activity decay of freely suspended cells incubated in solvent‐saturated aqueous media. Chloroform and n ‐decan‐1‐ol were the best choice of solvent, for both solubility and catalytic stability. High concentrations of water‐soluble additives, such as monosodium glutamate, were found to greatly improve the retention of activity in chloroform‐saturated media.