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Some considerations for optimization of desorption chromatography
Author(s) -
Gu Tingyue,
Tsai GowJen,
Tsao George T.
Publication year - 1991
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260370110
Subject(s) - desorption , adsorption , displacement chromatography , chromatography , chemistry , displacement (psychology) , column chromatography , langmuir adsorption model , nonlinear system , volumetric flow rate , analytical chemistry (journal) , chromatography column , thermodynamics , organic chemistry , physics , psychology , quantum mechanics , psychotherapist
Abstract The effect of isotherm parameters of a displacer on the efficiency of desorption Chromatography has been investigated numerically. A general nonlinear multicomponent rate equation model with Langmuir isotherm was used in this study. It was found that the best displacer in this kind of operation is usually not the one that is more strongly adsorbed than the adsorbates when the operation is to displace and concentrate the adsorbates from a saturated or partially saturated column and to minimize the amount of displacer used. The desorption Chromatography is different from the classical displacement development in both operational purpose and the requirement for the displacer. The desorption Chromatography in industrial practice was also analyzed and discussed for the case in which the displacer is introduced in either the same or the reverse flow direction after an incomplete frontal adsorption operation.