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A model for mercuric ion reduction in recombinant Escherichia coli
Author(s) -
Philippidis George P.,
Schottel Janet L.,
Hu WeiShou
Publication year - 1991
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260370108
Subject(s) - escherichia coli , chemistry , cytoplasm , recombinant dna , reductase , biophysics , reduction (mathematics) , biochemistry , enzyme , biology , gene , geometry , mathematics
Plasmid‐encoded mercuric reduction involves transfer of Hg 2+ across the cellular envelope and reduction to Hg 0 by the cytoplasmic mercuric reductase using NADPH. A mathematical model was developed for the binding and transfer of Hg 2+ by transport proteins and the subsequent reduction of Hg 2+ . The values of the model parameters were determined using experimental data. The derived rate expressions were similar to the previously experimentally determined ones. The model predicted that a differential amplification of the transport protein relative to mercuric reductase expression levels may enhance the Hg 2+ reduction rate in whole cells.