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Immobilization can improve the stability of hybridoma antibody productivity in serum‐free media
Author(s) -
Lee Gyun Min,
Palsson Bernhard O.
Publication year - 1990
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260361010
Subject(s) - productivity , laboratory flask , monoclonal antibody , antibody , chemistry , biology , immunology , economics , macroeconomics
Hybridoma cells (S3H5/γ2bA2) were cultivated in spinner flasks with 1% serum media and serum‐free media. Monoclonal antibody productivity was maintained in 1% serum media. However, cells in serum‐free media showed a decrease in antibody productivity, and it completely disappeared in IMDM‐based low protein medium. This loss of antibody productivity was not observed when the cells were immobilized in alginate beads. In fact, immobilization enhanced the specific MAb productivity.