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The large‐scale immobilization of Penicillium chrysogenum : Batch and continuous operation in an air‐lift reactor
Author(s) -
Keshavarz T.,
Eglin R.,
Walker E.,
Bucke C.,
Holt G.,
Bull A. T.,
Lilly M. D.
Publication year - 1990
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260360802
Subject(s) - penicillium chrysogenum , industrial fermentation , penicillin , chemistry , bioreactor , lift (data mining) , chromatography , biochemistry , fermentation , organic chemistry , antibiotics , computer science , data mining
A temperature‐sensitive cell division cycle mutant of Penicillium chrysogenum P2 has been immobilized on Celite and grown in a 250–320‐L working volume air‐lift fermenter. The ability to uncouple growth and penicillin synthesis by raising the temperature to 30 °C also overcame the problem of the free cell mass which appeared after 300 h operation with the parent organism. After 500 h operation, penicillin and ACV dimer were still being synthesized.