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A new biosensor for specific determination of glucose or fructose using an oxidoreductase of Zymomonas mobilis
Author(s) -
Park JeKyun,
Kim HakSung
Publication year - 1990
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260360713
Subject(s) - zymomonas mobilis , biosensor , fructose , chemistry , chromatography , oxidoreductase , biochemistry , enzyme , fermentation , ethanol fuel
Cells of Zymomonas mobilis were permeabilized with toluene in order to utilize the enzymes, glucose–fructose oxidoreductase and gluconolactonase, inside the intact cells. Permeabilized cells were immobilized in a gelatin membrane, and a whole cell enzyme electrode was constructed by fixing the membrane on pH electrode. The biosensor developed was used for specific determination of glucose or fructose by detecting the production rate of hydrogen ion. Optimum conditions for biosensor response were pH 6.2 and temperature of 39 °C. The biosensor was highly specific and reproducible, and calibration curves for glucose and fructose were excellent, being linear up to 5 and 50 g/L, respectively.