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Indirect electrochemical reduction of methemoglobin: Design of the process
Author(s) -
Labrune P.,
Bergel A.,
Comtat M.
Publication year - 1990
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260360402
Subject(s) - methemoglobin , reduction (mathematics) , electrochemistry , process (computing) , biochemical engineering , chemistry , computer science , engineering , mathematics , hemoglobin , electrode , biochemistry , geometry , operating system
Methemoglobin can be reduced on a platinum cathode using flavin mononucleotide as an oxido‐reduction mediator. The process requires the utilization of a filter‐press cell with compartments separated by a semi‐permeable membrane. Analysis of the various constraints imposed by the process itself and by the nature of the molecules involved shows that the electrolysis cell must operate at a low temperature, in strictly anaerobic conditions, in series with a storage tank, and with fluid circulation rates lower than approximately 0.8 m/s. A process has been designed that takes into account these imperatives and enables volumes of solution of the order of 200 cm 3 to be processed. It enables optimization of the flow rates used as well as of the methemoglobin/flavin ratio and is the forerunner of an industrial reactor.