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Prediction of the profiles of the elution bands of proteins in preparative liquid chromatography
Author(s) -
Katti Anita,
Huang JunXiong,
Guiochon Georges
Publication year - 1990
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260360311
Subject(s) - elution , chromatography , chemistry , aqueous solution , albumin , anion exchanger , high performance liquid chromatography , ion chromatography , ion exchange , phase (matter) , analytical chemistry (journal) , ion , biochemistry , organic chemistry
A semiequilibrium model of chromatography has been developed to account for the elution profile of high concentration bands. The use of this model requires the prior determination of the equilibrium isotherms of the studied compounds in the chromatographic phase system. The model is applied here to a study of the isocratic elution bands of chicken albumin and conalbumin on a weak anion exchanger in aqueous buffered solutions. Satisfactory agreement is observed between the experimental elution profiles of large‐sample‐size bands of chicken albumin and conalbumin.