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Inhibition of Trichoderma reesei cellulase by sugars and solvents
Author(s) -
Holtzapple Mark,
Cognata Mona,
Shu Yuancai,
Hendrickson Christie
Publication year - 1990
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260360310
Subject(s) - cellobiose , trichoderma reesei , cellulase , chemistry , acetone , ethanol , cellulose , butanol , non competitive inhibition , organic chemistry , beta glucosidase , biochemistry , enzyme
Inhibition of Trichoderma reesei cellulase by sugars (glucose, δ‐gluconolactone, and cellobiose) and solvents (ethanol, butanol, and acetone) was studied using cellulose azure. Glucose, cellobiose, ethanol, and butanol were noncompetitive inhibitors, δ‐gluconolactone was a mixed inhibitor, and acetone was a noncompetitive activator. Converting cellobiose to glucose reduces the effective inhibitor binding constant by 6 times and converting cellobiose to ethanol reduces it by 16 times.