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Effect of β‐cyclodextrin on production of L ‐phenylacetyl carbinol by immobilized cells of Saccharomyces cerevisiae
Author(s) -
Mahmoud Wafaa M.,
ElSayed AbdelHalim M. M.,
Coughlin Robert W.
Publication year - 1990
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260360307
Subject(s) - benzaldehyde , fermentation , saccharomyces cerevisiae , chemistry , cyclodextrin , substrate (aquarium) , biochemistry , transformation (genetics) , food science , yeast , catalysis , biology , gene , ecology
The rate and extent of microbial transformation of higher concentrations of benzaldehyde substrate to L ‐phenylacetyl carbinol (L‐PAC) by immobilized cells of Saccharomyces cerevisiae ATCC 834 was markedly stimulated by addition of different concentrations of β‐cyclodextrin (BCD) to the fermentation medium. With 0.5, 1.0, and 1.5% BCD in the fermentation medium and cumulative doses of benzaldehyde of 12 and 14 g/L, significantly higher yields of L‐PAC were obtained, about one‐ to twofold that of the yields of the control experiments. The favorable effects of BCD were evident in spite of its presence in stoichiometric concentrations significantly lower than those of benzaldehyde. The presence of BCD also appeared to stimulate microbial growth slightly. Enhanced cellular activity was reflected by faster D ‐glucose consumption and faster benzaldehyde utilization in the presence of BCD.