Premium
Macroporous and hydrophilic polymer resins modified with isothiocyanate groups for immobilization of enzymes
Author(s) -
Hasegawa Masanori,
Kitano Hiromi,
Nishida Ryosuke,
Kobashi Toshiyuki
Publication year - 1990
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260360302
Subject(s) - isothiocyanate , covalent bond , immobilized enzyme , chemistry , polymer , divinylbenzene , polymer chemistry , organic chemistry , chemical modification , enzyme , nucleophile , trypsin , alkaline phosphatase , styrene , catalysis , copolymer
Hydrophilic and macroporous polymer resins composed of glycerylmethacrylate, styrene, and divinylbenzene were quite easily modified with isothiocyanate groups using a Friedel–Crafts reaction with 3‐chloropropionyl chloride and subsequent nucleophilic reaction with KSCN. Alkaline phosphatase and trypsin could be covalently bound to the isothiocyanate‐carrying polymer resins, and the immobilized enzymes obtained were sufficiently active and stable due to a covalent bonding via a spacer group between the carrier resins and the enzymes and also due to a hydrophilic environment around the enzymes. A heterogeneity of the immobilized enzyme was taken into account to interpret the thermal denaturation process of the enzyme immobilized onto the resins.