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Studies on the deactivation of immobilized urease
Author(s) -
Vasudevan P. T.,
Ruggiano L.,
Weiland R. H.
Publication year - 1990
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260351110
Subject(s) - urease , chemistry , continuous stirred tank reactor , kinetics , immobilized enzyme , ammonia , product inhibition , chemical engineering , bioreactor , chromatography , combinatorial chemistry , enzyme , organic chemistry , non competitive inhibition , physics , quantum mechanics , engineering
Abstract The results of experiments in a fixed‐bed reactor and a CSTR containing urease immobilized on a nonporous support and conducted in the absence of diffusional limitations are reported. Kinetic parameters were established by separate batch experiments. The key observation was that the product ammonia attacked the free form of the enzyme and thereby illustrates the importance of mechanism in determining deactivation kinetics.