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Invertase‐catalyzed reactions in alcoholic solutions
Author(s) -
Selisko Barbara,
Ulbrich Renate,
Schellenberger Alfred,
Müller Ute
Publication year - 1990
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260351008
Subject(s) - invertase , chemistry , alkyl , hydrolysis , sucrose , fructose , methanol , ethanol , alcohol , propanol , organic chemistry , catalysis , aqueous solution
The formation of alkyl β‐ D ‐fructofuranosides by invertase from sucrose in aqueous solutions of methanol, ethanol, or n ‐propanol is studied for the dependence on alcohol and invertase concentrations as well as on reaction time. The yield of alkyl β‐ D ‐fructosides is shown to be controlled by three competitive reactions: the alcoholysis of sucrose, the hydrolysis of sucrose, and the hydrolysis of alkyl β‐ D ‐fructosides. Both the conversion rate of sucrose and the fraction of alkyl β‐ D ‐fructosides in the product mixture are dependent on the chain length of the alcohols. They decrease in the sequence methanol > ethanol > n ‐propanol. Alkyl β‐ D ‐fructosides are also formed by invertase starting from alcoholic solutions of fructose.