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Enzyme loading in a solid support with nonuniform pore size distribution
Author(s) -
Chiang C. L.,
Wu T. C.,
Wang Y. J.
Publication year - 1990
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260351004
Subject(s) - porosity , sigmoid function , immobilized enzyme , distribution (mathematics) , enzyme , enzyme assay , chemistry , materials science , radius , chromatography , composite material , mathematics , organic chemistry , computer science , mathematical analysis , machine learning , artificial neural network , computer security
The concept of pore size distribution is incorporated into the Clark model of enzyme immobilization in the present study. This refined model predicted that in the case of small harmonic pore radius with the same surface area and porosity of the support, more enzyme could be loaded in a support with nonuniform pores than that with uniform pores. In comparing the enzyme loading efficiency of the support with two different pore size distributions, the one with Gaussian distribution had the greater amount of enzyme immobilized than the other one with Rajagopalan's distribution. Furthermore, more enzyme could be loaded in a support with wider Gaussian pore size distribution than that with narrower distribution. The immobilized enzyme profile in the solid support with pore size distribution displayed a stepwise pattern which differed appreciably from the sigmoidal profile predicted for the support with uniform pore size. This stepwise enzyme distribution profile became sigmoidal with decreasing h T or increasing k . The new model could be used for designing protocols for an enzyme immobilization process.