Premium
Detection of microbial contamination in fermentation processes: Mass spectrometric determination of gram‐negative bacteria in Leuconostoc mesenteroides cultures
Author(s) -
Elmroth Ingrid,
Valeur Anders,
Odham Göran,
Larsson Lennart
Publication year - 1990
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260350806
Subject(s) - leuconostoc mesenteroides , enterobacter cloacae , chromatography , chemistry , fermentation , mass spectrometry , contamination , gas chromatography , bacteria , selected ion monitoring , gas chromatography–mass spectrometry , food science , enterobacteriaceae , biology , biochemistry , lactic acid , escherichia coli , ecology , genetics , gene
Gas chromatography/mass spectrometry was used to detect the presence of Enterobacter cloacae in cultures of Leuconostoc mesenteroides , an organism used in an industrial process for production of dextrane. The penta‐fluorobenzoyl‐methyl ester derivative of 3‐hydroxy‐myristic acid, a characteristic compound of gram‐negative bacteria, was used as the analyte. By using gas chromatography/mass spectrometry with selected ion monitoring, E. cloacae was determined over the range of 1 ppm to 1% in cultures of L. mesenteroides. The proposed analytical approach represents a useful alternative to conventional methods for determining contaminating organisms in industrial fermentation processes.