Optimum ph and temperature conditions for xylose fermentation by  Pichia stipitis | Zendy

Slininger P. J. | Zendy; Bothast R. J. | Zendy; Ladisch M. R. | Zendy; Okos M. R. | Zendy

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Optimum ph and temperature conditions for xylose fermentation by Pichia stipitis

Author(s) -

Slininger P. J.,

Bothast R. J.,

Ladisch M. R.,

Okos M. R.

Publication year - 1990

Publication title -

biotechnology and bioengineering

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.136

H-Index - 189

eISSN - 1097-0290

pISSN - 0006-3592

DOI - 10.1002/bit.260350710

Subject(s) - pichia stipitis , xylose , xylitol , fermentation , chemistry , yeast , ethanol , food science , ethanol fuel , biomass (ecology) , xylose metabolism , biochemistry , biology , agronomy

Pichia stipitis NRRL Y‐7124 is a xylose‐fermenting yeast able to accumulate ca. 57 g/L ethanol. Because optimum process conditions are important, data were collected to determine the effects of temperature and pH on growth and fermentation rates and product accumulations. Temperatures (26–35°C) providing optimum biomass and ethanol productivities did not necessarily provide maximum ethanol accumulation. Xylitol and residual xylose concentrations increased with temperature. Maximum ethanol selectivity was achieved at 25–26°C with minimal sacrifice to production rates. The temperature optimum for xylose could not be generalized to glucose fermentations, in which ethanol productivity and accumulation were optimum at 34°C. The optimum pH range for growth and fermentation on xylose was 4–7 at 25°C.

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