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Optimum ph and temperature conditions for xylose fermentation by Pichia stipitis
Author(s) -
Slininger P. J.,
Bothast R. J.,
Ladisch M. R.,
Okos M. R.
Publication year - 1990
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260350710
Subject(s) - pichia stipitis , xylose , xylitol , fermentation , chemistry , yeast , ethanol , food science , ethanol fuel , biomass (ecology) , xylose metabolism , biochemistry , biology , agronomy
Pichia stipitis NRRL Y‐7124 is a xylose‐fermenting yeast able to accumulate ca. 57 g/L ethanol. Because optimum process conditions are important, data were collected to determine the effects of temperature and pH on growth and fermentation rates and product accumulations. Temperatures (26–35°C) providing optimum biomass and ethanol productivities did not necessarily provide maximum ethanol accumulation. Xylitol and residual xylose concentrations increased with temperature. Maximum ethanol selectivity was achieved at 25–26°C with minimal sacrifice to production rates. The temperature optimum for xylose could not be generalized to glucose fermentations, in which ethanol productivity and accumulation were optimum at 34°C. The optimum pH range for growth and fermentation on xylose was 4–7 at 25°C.