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Asymmetric reduction of ketones with resting cells of Sulfolobus solfataricus
Author(s) -
Trincone Antonio,
Lama Licia,
Lanzotti Virginia,
Nicolaus Barbara,
De Rosa Mario,
Rossi Mosé,
Gambacorta Agata
Publication year - 1990
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260350603
Subject(s) - sulfolobus solfataricus , yield (engineering) , substrate (aquarium) , thermophile , sulfolobus , chemistry , organic chemistry , enzyme , materials science , biochemistry , biology , archaea , ecology , metallurgy , gene
The method of resting cells has been of interest in the development of biocatalysts applied to organic reactions. This article deals with the use of resting cells of a thermophilic archaebacterium Sulfolobus solfataricus , in the asymmetric reduction of acyclic, cyclic, and aromatic ketones. The system allows the continuous regeneration of endogenous coenzyme with the coupled substrate approach. The results indicate that the direction of hydride attack was equatorial on the re face of the carbonyl group of substrates producing (S)‐alcohols with a good optical yield. A convenient system for the reuse of resting cells has been set out to synthesize (S)‐alcohols on a preparative scale.