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Encapsulation of mammalian cell with chitosan‐CMC capsule
Author(s) -
Yoshioka Toshimitsu,
Hirano Ryogo,
Shioya Toshiaki,
Kako Masatoshi
Publication year - 1990
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260350110
Subject(s) - chitosan , capsule , chromatography , chemistry , cell culture , suspension culture , cell , monoclonal antibody , biophysics , antibody , biochemistry , immunology , biology , botany , genetics
Viable hybridoma cells were encapsulated. The capsules were formed in one step by placing a drop of cell suspension mixed with negatively charged carboxymethylcellulose (CMC) into a positively charged chitosan solution through the interpolymeric ionic interaction between two oppositely charged polymers. These capsules were found to have a mean diameter of about 1. 5 mm and wall thickness of 3 μm. The cells grew in the capsules using supplemented DMEM/F12 (four kinds of growth factor). The maximum cell density in encapsulating cell culture reached 1 × 10 7 cells/ml, 10 times higher than that obtained in the free cell culture. The maximum monoclonal antibody concentration in the free cell culture was 15μg/mL, but that in the capsule was 45μg/mL The antibody produced by the cell was concentrated about four times higher inside than outside of the capsules.