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Extraction of monocrotaline from Crotalaria spectabilis using supercritical carbon dioxide and carbon dioxide–ethanol mixtures
Author(s) -
Schaeffer Steven T.,
Zalkow Leon H.,
Teja Amyn S.
Publication year - 1989
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260341103
Subject(s) - solubility , supercritical carbon dioxide , supercritical fluid , supercritical fluid extraction , extraction (chemistry) , carbon dioxide , ethanol , chemistry , chromatography , organic chemistry
Monocrotaline, a pyrrolizidine alkaloid of chemotherapeutic interest, was successfully extracted from the crushed seeds of Crotalaria spectabilis using supercritical carbon dioxide and ethanol mixtures. Overall solubilities of the plant material in the supercritical fluid phase were as high as 1.1 mass percent, and monocrotaline solubilities were as high as 0.07 mass percent. The solubility of monocrotaline in the presence of other plant material was smaller by 50 to 98% compared with the solubility of pure monocrotaline in the supercritical fluid. Also, it was found that the extraction of the complex plant material was time‐dependent after approximately one percent of the original mass of the material had been extracted.