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Effects of paddle impeller geometry on power input and mass transfer in small‐scale animal cell culture vessels
Author(s) -
Aunins John G.,
Woodson Bruce A.,
Hale Timothy K.,
Wang Daniel I. C.
Publication year - 1989
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260340902
Subject(s) - impeller , microcarrier , paddle , mechanics , agitator , scale (ratio) , mass transfer , power (physics) , dissipation , suspension (topology) , suspension culture , mechanical engineering , scale up , process (computing) , materials science , chemistry , physics , mathematics , computer science , engineering , biology , thermodynamics , classical mechanics , cell , cell culture , genetics , operating system , biochemistry , quantum mechanics , homotopy , pure mathematics
Process scaleup for stirred‐tank animal cell cultures such as suspension and microcarrier cultures often begins at the bench scale in small spinner vessels. In order to initiate process development under the proper conditions, it is essential to know the physical conditions under which the cells are grown. In this article, power inputs and surface oxygen transfer rates to culture medium in 500‐mL Corning spinner vessels were determined as a function of the impeller geometry, impeller height, and agitation speed. The results obtained indicate that power dissipation dependency differs from literature correlations and may compromise scale up at constant power input from these vessels. These results are of general utility to researchers using small‐scale vessels.