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A new isolation and purification method for staphylococcal protein A using membrane encapsulated rabbit IgG‐agarose
Author(s) -
Sakoda Akiyoshi,
Wang Henry Y.
Publication year - 1989
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260340811
Subject(s) - agarose , rabbit (cipher) , isolation (microbiology) , chromatography , chemistry , membrane , microbiology and biotechnology , biology , biochemistry , statistics , mathematics
A new isolation and purification method for bioproducts using membrane‐encapsulated affinity adsorbents was investigated. The new method involves encapsulation of affinity adsorbents, batch adsorption of the bioproduct from whole fermentation broth and rapid batch desorption after dissolution of the capsule membranes. Recovery of protein A from Staphylococcus aureus was used as the model experimental system. Affinity adsorbents such as rabbit IgG‐agarose were successfully encapsulated within calcium alginate membranes and used directly to recover protein A from whole cell homogenate containing a number of macromolecular contaminants as well as suspended solids. Both high yield and high purity of protein A were recovered by this method in comparison with various previously reported methods.