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How suspension cultures of Catharanthus roseus respond to oxygen limitation: Small‐scale tests with applications to large‐scale cultures
Author(s) -
Snape J. B.,
Thomas N. H.,
Callow J. A.
Publication year - 1989
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260340806
Subject(s) - erlenmeyer flask , catharanthus roseus , oxygen , laboratory flask , biomass (ecology) , bioreactor , growth rate , limiting oxygen concentration , sugar , pulp and paper industry , suspension culture , biology , botany , chemistry , food science , chromatography , cell culture , agronomy , mathematics , geometry , organic chemistry , engineering , genetics
The effect of oxygen supply on the growth of suspension cultures of Catharanthus roseus in Erlenmeyer flasks was investigated. Below a critical oxygen supply rate the culture could not survive. By increasing the oxygen supply, a point is reached where the culture survives but no growth is possible. At higher oxygen supply rates there is a regime where both growth rate and the maximum biomass concentration increase with oxygen supply. Eventually there comes a point where no further increase in biomass is achieved, probably due to the depletion of the sugars; however, the growth rate continues to increase with oxygen supply until a maximum growth rate is obtained. The ratio of fresh to dry weight at maximum fresh weight increased with shaker table speed of rotation accompanied by a greater rate of sugar depletion.