How suspension cultures of  Catharanthus roseus  respond to oxygen limitation: Small‐scale tests with applications to large‐scale cultures | Zendy

Snape J. B. | Zendy; Thomas N. H. | Zendy; Callow J. A. | Zendy

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How suspension cultures of Catharanthus roseus respond to oxygen limitation: Small‐scale tests with applications to large‐scale cultures

Author(s) -

Snape J. B.,

Thomas N. H.,

Callow J. A.

Publication year - 1989

Publication title -

biotechnology and bioengineering

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.136

H-Index - 189

eISSN - 1097-0290

pISSN - 0006-3592

DOI - 10.1002/bit.260340806

Subject(s) - erlenmeyer flask , catharanthus roseus , oxygen , laboratory flask , biomass (ecology) , bioreactor , growth rate , limiting oxygen concentration , sugar , pulp and paper industry , suspension culture , biology , botany , chemistry , food science , chromatography , cell culture , agronomy , mathematics , geometry , organic chemistry , engineering , genetics

The effect of oxygen supply on the growth of suspension cultures of Catharanthus roseus in Erlenmeyer flasks was investigated. Below a critical oxygen supply rate the culture could not survive. By increasing the oxygen supply, a point is reached where the culture survives but no growth is possible. At higher oxygen supply rates there is a regime where both growth rate and the maximum biomass concentration increase with oxygen supply. Eventually there comes a point where no further increase in biomass is achieved, probably due to the depletion of the sugars; however, the growth rate continues to increase with oxygen supply until a maximum growth rate is obtained. The ratio of fresh to dry weight at maximum fresh weight increased with shaker table speed of rotation accompanied by a greater rate of sugar depletion.

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