Premium
Medium‐induced inhibition of microbial adsorption to nickel and activated charcoal
Author(s) -
Protheroe R. G.,
Cumming R. H.,
Matchett A.
Publication year - 1989
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260340703
Subject(s) - adsorption , activated charcoal , chemistry , yeast , desorption , chromatography , salt (chemistry) , yeast extract , charcoal , escherichia coli , growth medium , nuclear chemistry , biochemistry , bacteria , organic chemistry , fermentation , biology , genetics , gene
Equilibrium adsorption studies on Escherichia coli and Saccharomyces sp. revealed the capacity and affinity of these organisms for the surfaces of powdered charcoal and nickel. In simple salt solutions both organisms readily adsorbed to each solid with an affinity and maximum loading capacity individual to each cell–solid combination. In the presence of common growth media (lab‐lemco, nutrient broth, peptone, and yeast extract, individually at a concentration of 1.3%), each medium substantially inhibited adsorption. Each medium contained a protein‐aceous constituent as determined by ultraviolet (UV) analysis. The degree of inhibition was relative to medium concentration present during assay. Cell wall extracts from whole‐yeast cells also effectively inhibited adsorption. Cells adsorbed in the presence of sodium chloride solutions were susceptible to subsequent desorption by nutrient broth.