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Semicontinuous cultivation of Digitalis lanata cells: Production of β‐methyldigoxin in a 300‐L airlift bioreactor
Author(s) -
Reinhard E.,
Kreis W.,
Barthlen U.,
Helmbold U.
Publication year - 1989
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260340410
Subject(s) - airlift , bioreactor , biotransformation , chemistry , volume (thermodynamics) , substrate (aquarium) , digitalis , chromatography , botany , biology , biochemistry , enzyme , physics , ecology , quantum mechanics , medicine , heart failure
Selected Digitalis lanata cell lines cultivated in 1‐L shake flasks or 20‐L airlift bioreactors converted β‐methyldigitoxin into β‐methyldigoxin with almost no side reactions. This biotransformation process was optimized with regard to substrate supply and culture medium composition, and was then scaled up to a volume of 210 L using a 300‐L airlift bioreactor. A semicontinuous process was developed in which 513.3 g β‐methyldigoxin were produced after 89 days of cultivation.