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Lactose hydrolysis by immobilized β‐galactosidase in capillary bed reactor
Author(s) -
Peterson R. S.,
Hill C. G.,
Amundson C. H.
Publication year - 1989
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260340404
Subject(s) - lactose , beta galactosidase , hydrolysis , chemistry , chromatography , plug flow reactor model , capillary action , continuous stirred tank reactor , galactose , plug flow , packed bed , kinetics , biochemistry , materials science , thermodynamics , escherichia coli , physics , quantum mechanics , composite material , gene
The hydrolysis of lactose by immobilized β‐galactosidase was studied in a continuous‐flow capillary bed reactor operating at 30°C. Solutions containing 50, 100, and 150 g lactose and 0.5 g sodium acetate/L were fed to the reactor. Lactose conversions ranging from 24% to greater than 99% were achieved at reactor space times ranging from 0.06 to 6.3 min. These conversion data were successfully modeled in terms of a plug flow reactor model and a form of Michaelis–Menten kinetics which included competitive inhibition by both the alpha and beta forms of galactose.