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Biotransformation of acrylonitrile to acrylamide using immobilized whole cells of Brevibacterium CH1 in a recycle fed‐batch reactor
Author(s) -
Hwang Jun Sik,
Chang Ho Nam
Publication year - 1989
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260340311
Subject(s) - nitrile hydratase , acrylamide , acrylonitrile , chemistry , nitrile , amidase , immobilized enzyme , biotransformation , chromatography , yield (engineering) , nuclear chemistry , enzyme , organic chemistry , copolymer , materials science , polymer , metallurgy
Abstract Acrylamide was produced from acrylonitrile using immobilized Brevibacterium CH1 cells that were isolated from soil and found to possess nitrile hydratase activity. The reaction conditions and stability of the enzyme activity were studied. The conversion yield was nearly 100%, including a trace amount of acrylic acid. This strain showed strong activity of nitrile hydratase toward acrylonitrile and extremely low activity of amidase toward acrylamide. A packed bed reactor was operated in a fed‐batch manner for acrylamide production of high concentration. The acrylonitrile concentration was maintained below 3% and the operating temperature at 4°C to minimize enzyme deactivation.