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In vivo nuclear magnetic resonance analysis of immobilization effects on glucose metabolism of yeast Saccharomyces cerevisiae
Author(s) -
Galazzo Jorge L.,
Bailey James E.
Publication year - 1989
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260331009
Subject(s) - glycerol , fermentation , chemistry , fructose , metabolism , biochemistry , nuclear magnetic resonance spectroscopy , sugar phosphates , metabolite , saccharomyces cerevisiae , intracellular , yeast , phosphate , organic chemistry
Fermentation rates and intracellular compositions have been determined for alginate‐entrapped Saccharomyces cerevisiae and for identical cells in suspension. Glucose uptake and ethanol and glycerol production are approximately two times faster in immobilized cells than in suspended cells. Phosphorus‐31 nuclear magnetic resonance (NMR) spectroscopy of fermenting immobilized and suspended cells shows differences in intermediate metabolite levels such as fructose‐1,6 diphosphate, glucose‐6‐phosphate, and 3‐phosphoglycerate and in internal pH. Carbon‐13 NMR shows an increase in polysaccharide production. These data suggest that immobilization has accelerated the rate of glucose transport or of glucose phosphorylation. These effects of immobilization upon cell metabolism are observed in a very short period of time under conditions in which negligible DNA, RNA, or protein synthesis takes place.