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Selective adsorption/desorption of nucleic acids on submicron‐sized polymeric particles
Author(s) -
Kim Chan Wha,
Rha ChoKyun
Publication year - 1989
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260330917
Subject(s) - adsorption , chemistry , desorption , phosphate , nucleic acid , potassium , sodium , dna , potassium phosphate , chromatography , inorganic chemistry , nuclear chemistry , biochemistry , organic chemistry
DNA can be removed or separated by the selective adsorption/desorption on positively charged submicronsized polymeric particles (SSPP). The selective adsorption of DNA, in the presence of protein, on positively charged SSPP was accomplished by increasing the concentration of potassium phosphate or sodium phosphate. The adsorption of DNA was not affected by the concentration of potassium phosphate or sodium phosphate up to 1.2 M . On the other hand, the adsoprtion of a protein (bovine serum albumin) was completely impeded by 170m M potassium phosphate. DNA adsorbed on SSPP could be desorbed by increasing the concentration of NaCl or KCl, thus it can be recovered. DNA desorbed from SSPP when the concentration of NaCl or KC was higher than 0.6 M . A complete desorption of DNA was achieved at the concentration of NaCl or KCl above 1.2 M .