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Transient kinetics of hybridoma growth and monoclonal antibody production in serum‐limited cultures
Author(s) -
Dalili Mina,
Ollis David F.
Publication year - 1989
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260330807
Subject(s) - kinetics , growth rate , glutamine , monoclonal antibody , antibody , chemistry , yield (engineering) , biochemistry , biology , biophysics , thermodynamics , immunology , amino acid , mathematics , physics , geometry , quantum mechanics
Abstract A quantitative study of the influence of initial serum concentration on hybridoma growth rate, maximum viable and total cell yield, and specific antibody production rate is presented. The specific growth rate varied in a Monod fashion with initial serum levels (2–10% FCS), giving K m = 1.6 v/v% and μ max = 0.92 d −1 . The maximum cell yields (total and viable) were linear with initial serum level, indicating stoichiometric as well as kinetic limitation by serum component(s). The specific antibody production rate for each individual run fitted well to a non‐growth‐associated model. However, the non‐growth‐associated parameter varied monotonically with initial serum concentration, suggesting the catalytic role of serum component(s) in antibody production. Also, glutamine was utilized inefficiently, with at least a third of it secreted back into the culture supernate in the form of glutamate. While very simple model equations describe the specific growth and product formation rate for an individual batch run, the larger picture indicates need for a more detailed unstructured or structured model.