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Forced‐flow bioreactor for sucrose inversion using ceramic membrane activated by silanization
Author(s) -
Nakajima Mitsutoshi,
Watanabe Atsuo,
Jimbo Naoyuki,
Nishizawa Kouji,
Nakao Shinichi
Publication year - 1989
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260330709
Subject(s) - cross flow filtration , sucrose , ceramic membrane , membrane , silanization , chromatography , chemistry , invertase , chemical engineering , glutaraldehyde , phase inversion , membrane reactor , bioreactor , volumetric flow rate , porosity , materials science , biochemistry , organic chemistry , thermodynamics , physics , engineering
A forced‐flow enzyme membrane reactor system for sucrose inversion was investigated using three ceramic membranes having different pore sizes. Invertase was immobilized chemically to the inner surface of a ceramic membrane activated by a silane—glutaraldehyde technique. With the cross‐flow filtration of sucrose solution, the reaction rate was a function of the permeate flux, easily controlled by pressure. Using 0.5 μm support pore size of membrane, the volumetric productivity obtained was 10 times higher than that in a reported immobilized enzyme column reactor, with a short residence time of 5 s and 100% conversion of the sucrose inversion.