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Growth and death in overagitated microcarrier cell cultures
Author(s) -
Croughan Matthew S.,
Wang Daniel I. C.
Publication year - 1989
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260330611
Subject(s) - microcarrier , cell growth , microbiology and biotechnology , programmed cell death , cell , cell culture , replication (statistics) , biology , chemistry , biophysics , biochemistry , apoptosis , genetics , virology
The effects of hydrodynamic forces on cell growth are investigated for animal cells growing on microcarriers. A reduction in net growth was observed with high levels of agitation. DNA measurements indicated that the reduction in net growth was due entirely to cell death, from hydrodynamic forces. No inhibition or enhancement of cell replication appeared to occur with high levels of agitation.