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Kinetic model of hydrocortisone 1‐en‐dehydrogenation by Arthrobacter globiformis
Author(s) -
Gachok V. P.,
Grytsay V. I.,
Arinbasarova A. Yu.,
Medentsev A. G.,
Koshcheyenko K. A.,
Akimenko V. K.
Publication year - 1989
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260330602
Subject(s) - dehydrogenation , arthrobacter , hydrocortisone , chemistry , kinetic energy , chromatography , biochemistry , physics , catalysis , enzyme , quantum mechanics
A kinetic model of the hydrocortisone‐to‐prednisolone transformation by Arthrobacter globiformis is constructed using the experimental data obtained in studies of this process. Besides adequately describing experimental data, the model allows one to determine the relation between hydrocortisone oxidation and the level of endogenous substrates in bacterial cells, and the relation between the saturating concentration of hydrocortisone in the enzymic system of bacteria and the content of endogenous substrates in their cells, as well as the regulation of the transmembrane potential and the activation by the uncouplers.