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The transient responses of hybridoma cells to nutrient additions in continuous culture: II. Glutamine pulse and step changes
Author(s) -
Miller William M.,
Wilke Charles R.,
Blanch Harvey W.
Publication year - 1989
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260330414
Subject(s) - glutamine , alanine , metabolism , amino acid , biochemistry , serine , chemistry , ammonia , biology , oxygen , organic chemistry , enzyme
The transient and steady‐state responses of hybridoma growth and metabolism to glutamine pulse and step changes have been examined. Metabolic quotients are reported for oxygen, glucose, lactate, ammonia, glutamine, alanine, and other amino acids. The specific glutamine consumption rate increased rapidly after all glutamine additions, but the responses of the glucose and oxygen consumption rates and the cell concentration were found to depend on the intial feed glutamine concentration. The glucose consumption rate was 1.4‐10.9 times that of glutamine, and serine and branched‐chain amino acids were consumed in larger amounts at the higher glucose: glutamine uptake ratios. It was estimated that maintenance accounted for ca. 60% of the cellular ATP requirements at specific growth rates ranging from 0.57 to 0.68 day −1 .