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Surface immobilization of plant cells
Author(s) -
Archambault J.,
Volesky B.,
Kurz W. G. W.
Publication year - 1989
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260330307
Subject(s) - plant cell , biomass (ecology) , suspension (topology) , matrix (chemical analysis) , suspension culture , chemistry , plant tissue , bioreactor , chemical engineering , botany , biophysics , materials science , environmental science , pulp and paper industry , biology , cell culture , chromatography , biochemistry , agronomy , mathematics , engineering , genetics , homotopy , gene , pure mathematics
A novel technique has been developed to immobilize plant cells. The cells are deposited on a surface of manmade fibrous material that provides for strong binding of the plant tissue biomass growing in the submerged culture. The immobilized plant cells remain fully viable. Relatively uniform biomass loadings of up to 20 mg d.w. plant cells/cm 2 support material have been attained. All plant cells from the inoculum suspension became attached within the first 24–48 h depending on the support matrix configuration and hydraulic culture conditions. The advantages and scale‐up potential of this technique are discussed and compared to other culturing modes.