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Dynamics of foreign protein secretion from Saccharomyces cerevisiae
Author(s) -
Park Seujeung,
Ramirez W. Fred
Publication year - 1989
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260330305
Subject(s) - secretion , saccharomyces cerevisiae , yeast , heterologous , microbiology and biotechnology , secretory protein , cycloheximide , biology , mutant , biochemistry , chemistry , protein biosynthesis , gene
A mathematical model describing the dynamics of foreign protein secretion from yeast cells is developed. The secretion events, which are a series of complicated enzymatic reactions and carrier‐involved transport, are lumped to a practically applicable model structure, based on the major interactions between the heterologous polypeptides and the host cell's secretory machinery through the pathway. The developed model structure predicts that the secretion rate constant is represented as a saturated form with respect to the host cell's specific growth rate. The validity of the proposed model structure is tested by generating dynamic response data to a step input of cycloheximide. The model system used in the experiment is SEY2102‐s21, which has an integrated copy of a yeast secretion‐mutant invertase that simulates well typical gene cassettes designed to secrete mature foreign proteins utilizing the yeast cell's secretion signals. Protein quantification is done by gel electrophoresis followed by immuno‐blot on nitrocellulose filters and subsequent scanning with a reflectance densitometer. Experimental data confirm the proposed model structure.