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Mixed substrate‐metabolism in an obligate methanol utilizer: Chemostat studies
Author(s) -
Samuelov Nissim S.
Publication year - 1989
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260330211
Subject(s) - methylotroph , chemostat , obligate , methanol , carbon fibers , energy source , formic acid , chemistry , biochemistry , bacteria , biology , organic chemistry , botany , materials science , coal , composite number , composite material , genetics
In Pseudomonas C , an obligate RMP‐methylotroph, externally added formic acid and multi‐carbon intermediates are co‐metabolized in the obligate presence of methanol. Cell yield and methanol‐carbon conversion to cell mass are optimal in a chemostat culture growing on a mixture of energy sources and methanol as the sole limiting carbon source. Growth on methanol as the sole, obligate energy source and an additional carbon source (e.g. pyruvate) is less efficient. Under these energy limited conditions, the cyclic oxidation pathway is predominantly operated.