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A novel carbonochloridate for activation of supports containing hydroxyl groups
Author(s) -
Büttner W.,
Becker M.,
Rupprich Ch.,
Boeden H.F.,
Henklein P.,
Loth F.,
Dautzenberg H.
Publication year - 1989
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260330105
Subject(s) - chemistry , carbonate , hydrolysis , glycine , enzyme , organic chemistry , polymer chemistry , amino acid , biochemistry
The conditions for the introduction of active carbonate groups into supports containing hydroxyl groups by reaction with 5‐norbornene‐2.3‐dicarboximido carbonochloridate are described. Up to 1.5 mmol carbonate groups/g dry Sepharose 4B could be bound. In the case of glycine the reaction of the activated supports with the amino groups takes place with a 10‐fold higher rate than the hydrolysis of the carbonate groups, and high coupling yields can be reached. It is shown that the activated supports are well suitable for the preparation of carriers for affinity chromatography or the immobilization of enzymes.