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Serum‐free media in hybridoma culture and monoclonal antibody production
Author(s) -
Glassy Mark C.,
Tharakan John P.,
Chau Pao C.
Publication year - 1988
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260320809
Subject(s) - monoclonal antibody , secretion , antibody , hybridoma technology , monoclonal , chemically defined medium , biology , chemistry , microbiology and biotechnology , biochemistry , immunology , in vitro
The replacement of serum in hybridoma cultures is considered. The focus is on the effects of serum‐free media on hybridoma growth and monoclonal antibody secretion. Comparative literature data with serum supplemented cultures are discussed with an analysis of serum‐free formulations and selection rules for the serum‐free ingredients. In general, serum‐free media which are “lipid rich” can sustain cell growth rates approaching that of serum supplemented cultures. Specific antibody secretion rate, however, is usually higher in serum‐free media, irrespective of the lipid content.