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Polyelectrolyte precipitation of proteins: II. Models of the particle size distributions
Author(s) -
Fisher Rod R.,
Glatz Charles E.
Publication year - 1988
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260320610
Subject(s) - breakage , coalescence (physics) , shear rate , particle aggregation , particle (ecology) , population balance equation , brownian motion , particle size , shear (geology) , aggregate (composite) , chemistry , thermodynamics , population , chemical physics , materials science , mechanics , statistical physics , physics , viscosity , nanotechnology , mathematics , composite material , statistics , geology , oceanography , demography , sociology , astrobiology , nanoparticle
A population‐balance model has been used to characterize continuous polyelectrolyte precipitation of egg white proteins. We have modeled the particle size distributions of aggregates formed under a range of mixing conditions. The models, accounting for aggregate growth (by both shear‐driven and Brownian‐like collisions), breakage (by hydrodynamic shear or aggregate‐aggregate collisions), and birth (by the breakage of large aggregates), fit the data well. The kinetic constants show dependencies on shear rate and residence time that have not been previously theoretically predicted; these dependencies are due in part to aging effects on the aggregate. The model constants show a dominance of growth over breakage, supporting qualitative interpretations of the particle size distributions. A mechanism for growth‐rate enhancement, caused by polymer extensions from the particle surfaces, produced improved model performance. A collisional breakage mechanism is supported.