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Influence of immobilization procedure and salt environment on functional stability of chloroplast membranes: Experimental data and numerical analysis
Author(s) -
Thomasset Brigitte,
Thomas Daniel,
Lortie Robert
Publication year - 1988
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260320607
Subject(s) - glutaraldehyde , continuous stirred tank reactor , membrane , chemistry , ferricyanide , ionic strength , chemical engineering , thylakoid , matrix (chemical analysis) , chloroplast , chromatography , inorganic chemistry , biochemistry , organic chemistry , aqueous solution , engineering , gene
The interactions between chloroplast membranes and their microenvironment within artificial matrices (albumin‐glutaraldehyde matrix, polyurethane foam) where investigated. Particularly, the influence of a high‐ionic‐strength medium (0.75 M potassium citrate) on the stability of the photosynthetic ferricyanide reduction by immobilized thylakoids has been studied. A method of data analysis based on a nonlinear identification method combined with the numerical integration of the equation of the transient state of the continuous stirred tank reactor (CSTR) is proposed to estimate the actual degradation of the photosynthetic electron transfer. A statistical analysis achieved on the parameter values has allowed a quantitative assessment of the global behavior of immobilized chloroplast membranes. From the mathernatical analysis of the experimental data, we demonstrate that citrate used in the reaction media prevents the photoinactivation of the electron transfer chain whatever the nature of the matrix or the type of the reactor. The use of an albumin‐glutaraldehyde matrix or an open reactor during experiments also has allowed a better stabilization of the photosystems under operational conditions.